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Samples were stored at 20C for nitrite and leptin assays and at 80C for ghrelin and interleukin-6 (IL-6) analyses

Although cuprizone administration did not result in statistically significant shifts in gut microbial composition, distinct trends emerged

5-Amino-1MQ is a selective NNMT (nicotinamide N-methyltransferase) inhibitor that boosts NAD+ levels, reduces fat cell size, and enhances metabolic function

The search was restricted to articles in the English language, studies performed on human subjects, but were not limited by study design

Further, there were no significant changes in laboratory parameters, vital signs, or ECGs during the study in any treatment group, including placebo